Asian J Beauty Cosmetol. 2013; 11(3): 447-452.
Anti-obese Function of Selenate, An Essential Micronutrient, by Regulation of Adipogenesis in C3H10T1/2 Cells
ABSTRACT
Adipogenesis was defined as a differentiation process of preadipocytes into adipocytes and its regulation is crucial in obesity prevention. Several transcriptional factors such as peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer binding protein family (C/EBPs) were known as a key factors in early stage of adipogenesis. Selenium, an essentional micronutrient, has been reported to possess nurmerous biological activities. However, anti-obese function of selenium, particularly selenate, is relatively unknown. In this study, we investigated that inhibitory activities of selenate against adipocytes differentiation of C3H10T1/2 cells. Selenate treatment showed no cytotoxicity up to 100 μM in C3H10T1/2 cells. It showed dose-dependent suppression of adipogenesis between 1 to 50 μM. We also found that dose-dependently inhibited adipogenesis of C3H10T1/2 cells accompanied by reduced expression levels of PPARγ and C/ EBPβ as judged by PCR analysis. Our results imply that the inhibitory function of selenate is associated with the suppression of adipogenic genes in early stage of adipogenesis. These scientific evidences suggest that selenate could be used in development of obesity and/or overweight-related nutrient supplement and nutricosmetics.
Keywords :
Selenium, Selenate, Obesity, Adipogenesis, C3H10T1/2
