Asian J Beauty Cosmetol. 2014; 12(6): 945-951.

A study on Cosmetic Applications of Ethanol Extracts of Leonurus sibiricus L.

Bo-Ae Kim, and Young-Ah Jang

We conducted this study to investigate antioxidant and cytotoxicity of ethanol extracts of Leonurus sibiricus L. Also, we examined the cytoprotected effect of ethanol extracts of Leonurus sibiricus L. about antihemolytic of Sprague Dawley rat red blood cell. After extracting Leonurus sibiricus L. with ethanol. We treated samples by concentration and conducted DPPH radical scavenging activity and SOD-like activity. About human skin fibroblast HS68 cell, through MTT assay, we assessed toxicity of samples. Also, to assess effect of antihemolytic, we observed effect of extracts which we got after inducing oxidation of Sprague Dawley rat red blood cell using H₂O₂. The result showed that extracts of Leonurus sibiricus L. was effective as concentration of DPPH radical scavenging activity and SOD-like activity increased. After we checked cytotoxicity of Leonurus sibiricus L. using human fibroblast HS68 cell, it showed life rate more than 90 percent at 5~100 μg/ml and we made sure of its safety for skin. In the experiment for assessing of anti-hemolytic, we could observe the group that treated with extracts showed the higher rate of anti-hemolytic than control group that treated with H₂O₂ only. This result showed by eliminating ROS, extracts of Leonurus sibiricus L. could protect cell membrane and work as antioxidants. These results were that extracts of Leonurus sibiricus L. can eliminate active oxygen specie, have no cytotoxicity in fibroblast cell and show actual effectiveness of cell protection about anti-hemolytic activity in red blood cell, so they show possibility as functional natural product.

Keywords : Leonurus sibiricus L., Antioxidant, Cytotoxicity, Anti-hemolytic