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Kor J Aesthet Cosmetol > Volume 10(3); 2012 > Article
Kor J Aesthet Cosmetol 2012;10(3): 541-547.
H2O2로 산화적 스트레스가 유도된 HaCaT Keratinocytes에서 Genistein의 항산화 활성
김의수, 김종식, 김교남
Anti-oxidant Function of Genistein against H2O2-induced Oxidative Stress in HaCaT Keratinocytes
Eui-Su Kim, Jong Sik Kim, Gyo-Nam Kim
The nutri-cosmetics was defined as a nutraceuticals which can exert the function, beauty and the structure of the skin. Genistein has been reported to possess potent pro-apoptotic, anti-inflammatory, and anti-oxidant properties. However, the physiological role and its underlying molecular mechanisms of genistein in H2O2- treated HaCaT keratinocytes are unknown. In this study, we examined the cellular anti-oxidant activity of genistein against H2O2-induced oxidative stress in HaCaT keratinocytes. The cellular anti-oxidant activity of genistein was assessed in HaCaT cells as judged by the 2’7’-dichlorofluorescein-diacetate (DCFH-DA)- based assay and image analysis using fluorescence microscopy. Genistein dose-dependently and effectively ameliorate the H2O2-induced oxidative stress of HaCaT keratinocytes. We further revealed that the cellular anti-oxidant activity of genistein mediated by activating of nuclear factor erythroid-2-related factor 2 (Nrf2), the central transcriptional factor for expression of phase II detoxifying enzymes such as heme oxygenase-1 (HO- 1). Taken together, our results indicate that the activation of Nrf2 signaling is required for cellular anti-oxidant activity of genistein. These findings provide the scientific evidences for the development of Nrf2 targeted nutricosmetics.
Key words: Genistein, Nutri-cosmetics, Anti-oxidant activity, HaCaT keratinocytes, Nuclear factor erythroid-2-related factor 2
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