Asian J Beauty Cosmetol. 2012; 10(2): 345-352.
Effects of In Vitro Antioxidant and In Vivo Anti-aging Improvement of Finished Cosmetic Products Containing Ramalin
ABSTRACT
Although the studies of ingredient with antioxidant effect have been performing, in vitro and in vivo study of
product containing antioxidant was not sufficient relatively. Potential antioxidants are very unstable due to
auto-oxidation despite of their stabilized form exposed to air or sunlight. Especially, cosmetics were made
through the process of high temperature, homogenization and mixture of a lot of ingredients. So, we cannot
assure whether antioxidants keep the anti-oxidative efficacy on the finished product without any confirming
test. In this study, we investigated antioxidant effect through DPPH and ORAC method and human volunteer
test of three types of products with Actosome Ramalin as a stabilized form of Ramalin reported potent antioxidant
previously. IC50 value of finished products, Essence, Ample and Cream, was 6.7%, 41.62% and 3.2%
respectively. ORAC value showed 1034.52μM TE/g, 953.41μM TE/g and 1023.49μM TE/g. In in vivo human
test of Essence, skin hydration, texture, brightness and lifting were statistically improved after 3 weeks and 6
weeks compared to before using product. In the case of Ample, skin elasticity of R5 parameter on the side of
eye was statistically improved after 2 weeks and 4 weeks compared to before using product. Cream statistically
improved skin texture and skin tone after 2 weeks and 4 weeks. Any problem in the skin was not revealed in
human volunteer test. From these results, we suggest that we can claim the skin anti-oxidative effect of product
in the demonstration of anti-oxidative effect of finished product. And products formulated only antioxidant
as active ingredient were improved and alleviated phenomena of facial skin aging in human volunteer test.
Therefore, anti-oxidative efficacy demonstration of products containing anti-oxidant will be more practical and
realistic approach to measure anti-oxidative level than raw material test.
Keywords :
Ramalin Cosmetics, Antioxidant, Anti-aging, Finished product, ORAC
